Prothrombin Houston: a dysprothrombin identifiable by crossed immunoelectrofocusing and abnormal Echis carinatus venom activation
نویسندگان
چکیده
منابع مشابه
Blood Coagulation Induced by Iranian Saw-Scaled Viper (Echis Carinatus) Venom: Identification, Purification and Characterization of a Prothrombin Activator
Objective(s): Echis carinatus is one of the venomous snakes in Iran. The venom of Iranian Echis carinatus is a rich source of protein with various factors affecting the plasma protein and blood coagulation factor. Some of these proteins exhibit types of enzymatic activities. However, other items are proteins with no enzymatic activity. Materials and Methods: In order to study the mechanism ...
متن کاملBlood Coagulation Induced by Iranian Saw-Scaled Viper (Echis Carinatus) Venom: Identification, Purification and Characterization of a Prothrombin Activator
OBJECTIVE(S) Echis carinatus is one of the venomous snakes in Iran. The venom of Iranian Echis carinatus is a rich source of protein with various factors affecting the plasma protein and blood coagulation factor. Some of these proteins exhibit types of enzymatic activities. However, other items are proteins with no enzymatic activity. MATERIALS AND METHODS In order to study the mechanism and ...
متن کاملPurification and characterization of a variant of human prothrombin: prothrombin Segovia.
A dysprothrombin designated prothrombin Segovia was isolated from the plasma of an individual with normal prothrombin antigen and prothrombin activity lesser than 25% of the control prothrombin activity. Activation by prothrombinase complex showed a lower amidolytic than clotting activity, which suggests a lesser generation of active intermediates than normal prothrombin. When prothrombin Segov...
متن کاملDifferentiation between proteolytic activation and autocatalytic conversion of human prothrombin. Activation of recombinant human prothrombin and recombinant D419N-prothrombin by snake venoms from Echis carinatus and Oxyuranus scutellatus.
Recombinant human prothrombin (r-prothrombin) and recombinant mutant prothrombin with active site Asp419 substituted by Asn (D419N-prothrombin) were expressed in recombinant CHO cells, isolated and purified from the fermentation supernatant. The r-Prothrombin and D419N-prothrombin were digested by both Echis carinatus venom and Oxyuranus scutellatus venom. Prior to, during and after activation,...
متن کاملblood coagulation induced by iranian saw-scaled viper (echis carinatus) venom: identification, purification and characterization of a prothrombin activator
objective(s): echis carinatus is one of the venomous snakes in iran. the venom of iranian echis carinatus is a rich source of protein with various factors affecting the plasma protein and blood coagulation factor. some of these proteins exhibit types of enzymatic activities. however, other items are proteins with no enzymatic activity. materials and methods: in order to study the mechanism an...
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ژورنال
عنوان ژورنال: Blood
سال: 1980
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v55.5.811.811